Part:BBa_K1432001:Design
A Synthetic microcystin-degrading Mlr A gene
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We will construct a mlr promoter-GPF-mlrA cluster and clone it into the E. coli-L. lactis shuttle expression vector. So it can be easily constructed in the laboratory and could avoid secondary pollution in the natural environment of E.coli. we synthesized mlrA gene, there are 336 codons in Sphingomonas species ACM-3962. We replace 246 of them in order to increase the expression of mlrA because they are not abundant codons in Lactococcus lactis. And we successfully did it both in E.coli and L.lactis. When we induced the expression of protein, it shows that L.lactis could be more efficiently expressed than E.coli.
The codons of Lactococcus lactis subsp. we used.
- TAA
A GCT C TGT D GAT E GAA F TTT G GGA H CAT I ATT K AAA L TTA M ATG N AAT P CCA Q CAA R CGA S TCA T ACT V GTT W TGG Y TAT
Source
Sphingomonas sp. ACM-3962
References
Environ Toxicol. 2001;16(6):523-34 Lactococcus lactis subsp. cremoris SK11 [gbbct]: 2504 CDS's (696252 codons) http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=272622